ZC3H12A (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	ZC3H12A (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9547
Supplier Catalog Number:	9547
Alternative Catalog Number:	PRS-9547-0.02,PRS-9547-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-ZC3H12A antibody (9547) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A. The immunogen is located within the last 50 amino acids of ZC3H12A.
Conjugation:	Unconjugated
Alternative Names:	ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocyte chemotactic protein-induced protein 1, MCP-induced protein 1, MCPIP-1, Zinc finger CCCH domain-containing protein 12A

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 66 kD Observed: 66 kD
NCBI:	80149
UniProt:	Q5D1E8
Buffer:	ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 2 µg mL, IF: 10 µg mL, IHC: 1 µg mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with 9547 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5. Immunohistochemistry Validation of ZC3H12A in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin
	Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1. WB Validation in Human Lung Loading: 15 &956,g of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 &956,g/mL Lane 2: 4 &956,g/mL
	Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: ZC3H12A 9547, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3. Western Blot Validation in Rat Tissues Loading: 15 &956,g of lysates per lane. Antibodies: ZC3H12A 9547, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.