ZC3H12A (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-9547
- Bilder (7)
| Artikelname: | ZC3H12A (CT) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-9547 |
| Hersteller Artikelnummer: | 9547 |
| Alternativnummer: | PRS-9547-0.02,PRS-9547-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-ZC3H12A antibody (9547) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A. The immunogen is located within the last 50 amino acids of ZC3H12A. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocyte chemotactic protein-induced protein 1, MCP-induced protein 1, MCPIP-1, Zinc finger CCCH domain-containing protein 12A |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 2 µg mL, IF: 10 µg mL, IHC: 1 µg mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples, Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested. |
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Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with 9547 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). |
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Figure 5. Immunohistochemistry Validation of ZC3H12A in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin |
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Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (9547) at 1 &956,g/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
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Figure 1. WB Validation in Human Lung Loading: 15 &956,g of lysate per lane. Antibodies: ZC3H12A 9547, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2 &956,g/mL Lane 2: 4 &956,g/mL |
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Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: ZC3H12A 9547, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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Figure 3. Western Blot Validation in Rat Tissues Loading: 15 &956,g of lysates per lane. Antibodies: ZC3H12A 9547, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. |
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