sRANK Ligand Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	sRANK Ligand Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3963
Supplier Catalog Number:	3963
Alternative Catalog Number:	PRS-3963-0.02,PRS-3963-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-sRANK-L antibody (3963) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.
Conjugation:	Unconjugated
Alternative Names:	sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 36kDObserved: 36 kD
NCBI:	8600
UniProt:	O14788
Buffer:	sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.25-4 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human and rat samples. All other applications and species not yet tested.

	Figure 4 Western Blot Validation in Rat Liver TissueLoading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (A: 0.25 &956,g/mL and B: 0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Immunohistochemistry Validation of sRANK-L in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (3963) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017) Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (3963) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.
	Figure 1 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 1 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human sRANK-L recombinant protein per lane.Antibodies: sRANK-L 3963 (A: 1 &956,g/mL, B: 2 &956,g/mL and C: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 27kD.