sRANK Ligand Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-3963
- Bilder (6)
| Artikelname: | sRANK Ligand Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-3963 |
| Hersteller Artikelnummer: | 3963 |
| Alternativnummer: | PRS-3963-0.02,PRS-3963-0.1 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Anti-sRANK-L antibody (3963) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 0.25-4 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human and rat samples. All other applications and species not yet tested. |
|
|
Figure 4 Western Blot Validation in Rat Liver TissueLoading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (A: 0.25 &956,g/mL and B: 0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 5 Immunohistochemistry Validation of sRANK-L in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (3963) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. |
|
|
Figure 6 Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017) Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (3963) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline. |
|
|
Figure 1 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 1 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: sRANK-L 3963 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. |
|
|
Figure 3 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human sRANK-L recombinant protein per lane.Antibodies: sRANK-L 3963 (A: 1 &956,g/mL, B: 2 &956,g/mL and C: 4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 27kD. |
Produktgarantie und fachkundiger Support
