SMAD2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-23-117
- Images (6)
| Article Name: | SMAD2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-23-117 |
| Supplier Catalog Number: | 23-117 |
| Alternative Catalog Number: | PRS-23-117-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SMAD2 (NP_001003652.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | SMAD2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2 |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
|
|
Western blot analysis of extracts of various cell lines, using SMAD2 antibody (23-117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
|
|
Western blot analysis of extracts from normal (control) and SMAD2 knockout (KO) HeLa cells, using SMAD2 antibody (23-117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
|
|
Immunohistochemistry of paraffin-embedded mouse spleen using SMAD2 antibody (23-117) at dilution of 1:100 (40x lens). |
|
|
Immunofluorescence analysis of HeLa cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
|
|
Immunofluorescence analysis of NIH/3T3 cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
|
|
Immunofluorescence analysis of PC-12 cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Product Guarantee and Expert Support
