SMAD2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-23-117
- Bilder (6)
| Artikelname: | SMAD2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-23-117 |
| Hersteller Artikelnummer: | 23-117 |
| Alternativnummer: | PRS-23-117-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SMAD2 (NP_001003652.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | SMAD2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using SMAD2 antibody (23-117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Western blot analysis of extracts from normal (control) and SMAD2 knockout (KO) HeLa cells, using SMAD2 antibody (23-117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
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Immunohistochemistry of paraffin-embedded mouse spleen using SMAD2 antibody (23-117) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC-12 cells using SMAD2 antibody (23-117) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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