Western blot analysis of extracts of various cell lines, using BCAS3 antibody (22-827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using BCAS3 antibody (22-827). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAS3 antibody (22-827). Western blot was performed from the immunoprecipitate using BCAS3 antibody (22-827) at a dilition of 1:1000.
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