BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-827
Artikelname: BCAS3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-827
Hersteller Artikelnummer: 22-827
Alternativnummer: PRS-22-827-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3).
Konjugation: Unconjugated
Alternative Synonym: BCAS3 Antibody: MAAB, GAOB1, Breast carcinoma-amplified sequence 3
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 115kDa
NCBI: 54828
UniProt: Q9H6U6
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using BCAS3 antibody (22-827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (22-827) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using BCAS3 antibody (22-827). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAS3 antibody (22-827). Western blot was performed from the immunoprecipitate using BCAS3 antibody (22-827) at a dilition of 1:1000.