mH2A1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-656
Article Name: mH2A1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-656
Supplier Catalog Number: 22-656
Alternative Catalog Number: PRS-22-656-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human H2AFY (NP_613258.2).
Conjugation: Unconjugated
Alternative Names: H2AFY, H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 40kDa
NCBI: 9555
UniProt: O75367
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using H2AFY antibody (22-656) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using mH2A1 antibody (22-656). Blue: DAPI for nuclear staining.