Western blot analysis of extracts of various cell lines, using H2AFY antibody (22-656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using mH2A1 antibody (22-656) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using mH2A1 antibody (22-656). Blue: DAPI for nuclear staining.
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