CCT4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-304
Article Name: CCT4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-304
Supplier Catalog Number: 22-304
Alternative Catalog Number: PRS-22-304-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).
Conjugation: Unconjugated
Alternative Names: CCT4, SRB, Cctd, CCT-DELTA
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 60kDa
NCBI: 10575
UniProt: P50991
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CCT4 antibody (22-304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat stomach using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CCT4 antibody (22-304). Blue: DAPI for nuclear staining.