Western blot analysis of extracts of various cell lines, using CCT4 antibody (22-304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat stomach using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CCT4 antibody (22-304) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CCT4 antibody (22-304). Blue: DAPI for nuclear staining.
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