HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-004
Article Name: HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-004
Supplier Catalog Number: 22-004
Alternative Catalog Number: PRS-22-004-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2).
Conjugation: Unconjugated
Alternative Names: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
NCBI: 1994
UniProt: Q15717
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (22-004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (22-004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HuR / ELAVL1 antibody (22-004) at dilution of 1:100. Blue: DAPI for nuclear staining.