HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-22-004
- Bilder (6)
| Artikelname: | HuR / ELAVL1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-22-004 |
| Hersteller Artikelnummer: | 22-004 |
| Alternativnummer: | PRS-22-004-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using HuR / ELAVL1 antibody (22-004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s. |
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Western blot analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR / ELAVL1 antibody (22-004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
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Immunohistochemistry of paraffin-embedded human esophagus using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human stomach using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using HuR / ELAVL1 antibody (22-004) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of U2OS cells using HuR / ELAVL1 antibody (22-004) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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