EIF4B Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-645
Article Name: EIF4B Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-645
Supplier Catalog Number: 19-645
Alternative Catalog Number: PRS-19-645-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Conjugation: Unconjugated
Alternative Names: EIF4B, EIF-4B, PRO1843
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 80kDa
NCBI: 1975
UniProt: P23588
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EIF4B antibody (19-645) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4B antibody (19-645). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug EIF4B antibody (19-645). Western blot was performed from the immunoprecipitate using EIF4B antibody (19-645) at a dilition of 1:1000.