EIF4B Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-19-645
- Bilder (6)
| Artikelname: | EIF4B Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-19-645 |
| Hersteller Artikelnummer: | 19-645 |
| Alternativnummer: | PRS-19-645-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | EIF4B, EIF-4B, PRO1843 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using EIF4B antibody (19-645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
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Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of A549 cells using EIF4B antibody (19-645). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug EIF4B antibody (19-645). Western blot was performed from the immunoprecipitate using EIF4B antibody (19-645) at a dilition of 1:1000. |
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