HHLA2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-877
Article Name: HHLA2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-877
Supplier Catalog Number: 14-877
Alternative Catalog Number: PRS-14-877-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human HHLA2 (NP_009003.1).
Conjugation: Unconjugated
Alternative Names: HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 45-50kDa
NCBI: 11148
UniProt: Q9UM44
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HHLA2 antibody (14-877) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).