Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HHLA2 antibody (14-877) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HHLA2 antibody (14-877) at dilution of 1:100 (40x lens).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten