ECH1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-14-714
Article Name: ECH1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-14-714
Supplier Catalog Number: 14-714
Alternative Catalog Number: PRS-14-714-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Conjugation: Unconjugated
Alternative Names: ECH1, HPXEL
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 36kDa
NCBI: 1891
UniProt: Q13011
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).