ECH1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-714
Artikelname: ECH1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-714
Hersteller Artikelnummer: 14-714
Alternativnummer: PRS-14-714-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Konjugation: Unconjugated
Alternative Synonym: ECH1, HPXEL
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 36kDa
NCBI: 1891
UniProt: Q13011
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ECH1 Antibody (14-714) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody (14-714) at dilution of 1:150 (40x lens).