HSPA9 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-13-203
Article Name: HSPA9 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-13-203
Supplier Catalog Number: 13-203
Alternative Catalog Number: PRS-13-203-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human HSPA9 (NP_004125.3).
Conjugation: Unconjugated
Alternative Names: HSPA9, Heat shock 70 kDa protein 9, GRP-75, Heat shock 70kD protein 9B, GRP75, Mortalin-2, Mortalin, perinuclear, Mot-2, MOT2, Peptide-binding protein 74, MOT, MTHSP75, p66-mortalin, PBP74, CSA, HSPA9B, Mortalin
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 80kDa
NCBI: 3313
UniProt: P38646
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HSPA9 antibody (13-203). Blue: DAPI for nuclear staining.