HSPA9 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-203
Artikelname: HSPA9 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-203
Hersteller Artikelnummer: 13-203
Alternativnummer: PRS-13-203-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human HSPA9 (NP_004125.3).
Konjugation: Unconjugated
Alternative Synonym: HSPA9, Heat shock 70 kDa protein 9, GRP-75, Heat shock 70kD protein 9B, GRP75, Mortalin-2, Mortalin, perinuclear, Mot-2, MOT2, Peptide-binding protein 74, MOT, MTHSP75, p66-mortalin, PBP74, CSA, HSPA9B, Mortalin
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 80kDa
NCBI: 3313
UniProt: P38646
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HSPA9 antibody (13-203) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HSPA9 antibody (13-203) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HSPA9 antibody (13-203). Blue: DAPI for nuclear staining.