Western blot analysis of extracts of U-937 cells, using CD4 antibody (13-152) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.
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