CD4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-13-152
Artikelname: CD4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-13-152
Hersteller Artikelnummer: 13-152
Alternativnummer: PRS-13-152-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1).
Konjugation: Unconjugated
Alternative Synonym: CD4 Antibody [9H5A8] : CD4mut
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 51kDa
NCBI: 920
UniProt: P01730
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of U-937 cells, using CD4 antibody (13-152) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody (13-152) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody (13-152) at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using GRP78 / BiP antibody (13-101) at dilution of 1:200. Blue: DAPI for nuclear staining.