NUMA1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA016185YA01HU
- Images (4)
| Article Name: | NUMA1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA016185YA01HU |
| Supplier Catalog Number: | CSB-PA016185YA01HU |
| Alternative Catalog Number: | CSB-PA016185YA01HU-100UG, CSB-PA016185YA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Centrophilin stabilizes mitotic spindle in mitotic cells antibody, NMP 22 antibody, Nuclear matrix protein 22 antibody, Nuclear mitotic apparatus protein 1 antibody, Nuclear mitotic apparatus protein antibody, NUMA 1 antibody, NUMA antibody, NuMA protein antibody, NUMA1 antibody, NUMA1_HUMAN antibody, SP H antigen antibody, SP-H antigen antibody, Structural nuclear protein antibody |
| Clonality: | Polyclonal |
| UniProt: | Q14980 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | NUMA1 |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA016185YA01HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA016185YA01HU at dilution of 1:100 |
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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: NUMA1 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 239, 237, 202, 201, 110 kDa Observed band size: 239 kDa |
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Immunofluorescence staining of MCF-7 cells with CSB-PA016185YA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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