NUMA1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA016185YA01HU
Artikelname: NUMA1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA016185YA01HU
Hersteller Artikelnummer: CSB-PA016185YA01HU
Alternativnummer: CSB-PA016185YA01HU-100UG, CSB-PA016185YA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: Centrophilin stabilizes mitotic spindle in mitotic cells antibody, NMP 22 antibody, Nuclear matrix protein 22 antibody, Nuclear mitotic apparatus protein 1 antibody, Nuclear mitotic apparatus protein antibody, NUMA 1 antibody, NUMA antibody, NuMA protein antibody, NUMA1 antibody, NUMA1_HUMAN antibody, SP H antigen antibody, SP-H antigen antibody, Structural nuclear protein antibody
Klonalität: Polyclonal
UniProt: Q14980
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: NUMA1
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA016185YA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA016185YA01HU at dilution of 1:100
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: NUMA1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 239, 237, 202, 201, 110 kDa
Observed band size: 239 kDa
Immunofluorescence staining of MCF-7 cells with CSB-PA016185YA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).