HIST1H2BB Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010402HA01HU
- Images (4)
| Article Name: | HIST1H2BB Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010402HA01HU |
| Supplier Catalog Number: | CSB-PA010402HA01HU |
| Alternative Catalog Number: | CSB-PA010402HA01HU-100UG, CSB-PA010402HA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | HIST1H2BB antibody, H2BFFHistone H2B type 1-B antibody, Histone H2B.1 antibody, Histone H2B.f antibody, H2B/f antibody |
| Clonality: | Polyclonal |
| UniProt: | P33778 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | HIST1H2BB |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500 |
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Immunofluorescent analysis of PC-3 cells using CSB-PA010402HA01HU at dilution of 1:266 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: Mouse liver tissue, Rat liver tissue All lanes: HIST1H2BB antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
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