HIST1H2BB Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA010402HA01HU
Artikelname: HIST1H2BB Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA010402HA01HU
Hersteller Artikelnummer: CSB-PA010402HA01HU
Alternativnummer: CSB-PA010402HA01HU-100UG, CSB-PA010402HA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Konjugation: Unconjugated
Alternative Synonym: HIST1H2BB antibody, H2BFFHistone H2B type 1-B antibody, Histone H2B.1 antibody, Histone H2B.f antibody, H2B/f antibody
Klonalität: Polyclonal
UniProt: P33778
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: HIST1H2BB
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500
Immunofluorescent analysis of PC-3 cells using CSB-PA010402HA01HU at dilution of 1:266 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot
Positive WB detected in: Mouse liver tissue, Rat liver tissue
All lanes: HIST1H2BB antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa