Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA36acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Immunocytochemistry analysis of Hela cells using CSB-PA010389PA36acHU at dilution of 1:100
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010389PA36acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h) All lanes: HIST1H2AG antibody at 1µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa
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