Acetyl-HIST1H2AG (K36) Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA010389PA36ACHU
Artikelname: Acetyl-HIST1H2AG (K36) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA010389PA36ACHU
Hersteller Artikelnummer: CSB-PA010389PA36acHU
Alternativnummer: CSB-PA010389PA36ACHU-100UL, CSB-PA010389PA36ACHU-50UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, ELISA, ICC, IF, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody
Klonalität: Polyclonal
UniProt: P0C0S8
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: HIST1H2AG
Application Verdünnung: Recommended dilution: WB:1:200-1:2000, ICC:1:20-1:200, IF:1:50-1:200
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA36acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Immunocytochemistry analysis of Hela cells using CSB-PA010389PA36acHU at dilution of 1:100
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010389PA36acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h)
All lanes: HIST1H2AG antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa