HARS Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA010137ESR2HU
- Images (4)
| Article Name: | HARS Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA010137ESR2HU |
| Supplier Catalog Number: | CSB-PA010137ESR2HU |
| Alternative Catalog Number: | CSB-PA010137ESR2HU-100UL, CSB-PA010137ESR2HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | cytoplasmic antibody, EC 6.1.1.21 antibody, FLJ20491 antibody, HARS antibody, HisRS antibody, Histidine tRNA ligase, cytoplasmic antibody, histidine translase antibody, Histidine tRNA ligase antibody, Histidine--tRNA ligase antibody, Histidyl tRNA synthetase antibody, Histidyl-tRNA synthetase antibody, HRS antibody, Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody, SYHC_HUMAN antibody, USH3B antibody |
| Clonality: | Polyclonal |
| UniProt: | P12081 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | HARS |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA010137ESR2HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA010137ESR2HU at dilution of 1:100 |
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Western blot All lanes: HARS antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 53, 51, 55 kDa Observed band size: 58 kDa |
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Immunoprecipitating HARS in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA010137ESR2HU in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA010137ESR2HU (4ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) |
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