HARS Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010137ESR2HU
- Bilder (4)
| Artikelname: | HARS Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010137ESR2HU |
| Hersteller Artikelnummer: | CSB-PA010137ESR2HU |
| Alternativnummer: | CSB-PA010137ESR2HU-100UL, CSB-PA010137ESR2HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | cytoplasmic antibody, EC 6.1.1.21 antibody, FLJ20491 antibody, HARS antibody, HisRS antibody, Histidine tRNA ligase, cytoplasmic antibody, histidine translase antibody, Histidine tRNA ligase antibody, Histidine--tRNA ligase antibody, Histidyl tRNA synthetase antibody, Histidyl-tRNA synthetase antibody, HRS antibody, Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody, SYHC_HUMAN antibody, USH3B antibody |
| Klonalität: | Polyclonal |
| UniProt: | P12081 |
| Puffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HARS |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA010137ESR2HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA010137ESR2HU at dilution of 1:100 |
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Western blot All lanes: HARS antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 53, 51, 55 kDa Observed band size: 58 kDa |
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Immunoprecipitating HARS in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA010137ESR2HU in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA010137ESR2HU (4ug) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) |
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