GJC1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009458EA01HU
Article Name: GJC1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009458EA01HU
Supplier Catalog Number: CSB-PA009458EA01HU
Alternative Catalog Number: CSB-PA009458EA01HU-100UG, CSB-PA009458EA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: Connexin 45 / GJA7 antibody, Connexin 45 antibody, Connexin-45 antibody, Connexin45 antibody, CX 31.9 antibody, CX 45 antibody, CX31.9 antibody, Cx45 antibody, CXG1_HUMAN antibody, DKFZp686P0738 antibody, Gap junction alpha 7 protein antibody, Gap junction alpha-7 protein antibody, Gap junction gamma 1 protein antibody, Gap junction gamma-1 protein antibody, Gap junction protein alpha 7 45kDa antibody, Gap junction protein alpha 7 antibody, Gap junction protein, gamma 1, 45kDa antibody, GJA 7 antibody, GJA7 antibody, GJC1 antibody, GJD3 antibody
Clonality: Polyclonal
UniProt: P36383
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: GJC1
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:20-1:100
Western Blot
Positive WB detected in: SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), Hela whole cell lysate(30µg), HT29 whole cell lysate(30µg),293T whole cell lysate(30µg),Mouse Heart tissue lysate30µg)
All lanes: GJC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 30s
Immunofluorescence staining of Hela cell with CSB-PA009458ESR1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).