Western Blot Positive WB detected in: SY5Y whole cell lysate(30µg), K562 whole cell lysate(30µg), Hela whole cell lysate(30µg), HT29 whole cell lysate(30µg),293T whole cell lysate(30µg),Mouse Heart tissue lysate30µg) All lanes: GJC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 30s
Immunofluorescence staining of Hela cell with CSB-PA009458ESR1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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