GIPR Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009438ESR1HU
Article Name: GIPR Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009438ESR1HU
Supplier Catalog Number: CSB-PA009438ESR1HU
Alternative Catalog Number: CSB-PA009438ESR1HU-100UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: Gastric inhibitory polypeptide receptor antibody, GIP R antibody, GIP-R antibody, Gippr antibody, Gipr antibody, GIPR_HUMAN antibody, Glucose dependent insulinotropic polypeptide receptor antibody, Glucose-dependent insulinotropic polypeptide receptor antibody, Gm1081 antibody, Gm160 antibody, INSULINOTROPIC POLYPEPTIDE RECEPTOR, GLUCOSE DEPENDENT antibody, MGC126722 antibody, PGQTL2 antibody, RATGIPPR antibody
Clonality: Polyclonal
UniProt: P48546
Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Purity: Antigen Affinity Purified
Form: Liquid
Target: GIPR
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:20-1:100
IHC image of CSB-PA822219LA01HU diluted at 1:20 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: Hela whole cell lysate(20µg),A549 whole cell lysate(20µg), K562 whole cell lysate(20µg), SY5Y whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: GIPR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 54,49,50 kDa
Observed band size: 54 kDa
Exposure time: 10s