GIPR Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA009438ESR1HU
Artikelname: GIPR Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA009438ESR1HU
Hersteller Artikelnummer: CSB-PA009438ESR1HU
Alternativnummer: CSB-PA009438ESR1HU-100UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: Gastric inhibitory polypeptide receptor antibody, GIP R antibody, GIP-R antibody, Gippr antibody, Gipr antibody, GIPR_HUMAN antibody, Glucose dependent insulinotropic polypeptide receptor antibody, Glucose-dependent insulinotropic polypeptide receptor antibody, Gm1081 antibody, Gm160 antibody, INSULINOTROPIC POLYPEPTIDE RECEPTOR, GLUCOSE DEPENDENT antibody, MGC126722 antibody, PGQTL2 antibody, RATGIPPR antibody
Klonalität: Polyclonal
UniProt: P48546
Puffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: GIPR
Application Verdünnung: Recommended dilution: WB:1:1000-1:3000, IHC:1:20-1:100
IHC image of CSB-PA822219LA01HU diluted at 1:20 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: Hela whole cell lysate(20µg),A549 whole cell lysate(20µg), K562 whole cell lysate(20µg), SY5Y whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
All lanes: GIPR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 54,49,50 kDa
Observed band size: 54 kDa
Exposure time: 10s