GABRA4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009143LA01HU
Article Name: GABRA4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009143LA01HU
Supplier Catalog Number: CSB-PA009143LA01HU
Alternative Catalog Number: CSB-PA009143LA01HU-100UG, CSB-PA009143LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: GABA(A) receptor subunit alpha 4 antibody, GABA(A) receptor subunit alpha-4 antibody, GABR A4 antibody, GABRA 4 antibody, Gabra4 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 4 antibody, Gamma aminobutyric acid A receptor alpha 4 antibody, Gamma aminobutyric acid receptor alpha 4 subunit antibody, Gamma aminobutyric acid receptor subunit alpha 4 antibody, Gamma-aminobutyric acid receptor subunit alpha-4 antibody, GBRA4_HUMAN antibody
Clonality: Polyclonal
UniProt: P48169
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: GABRA4
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:20-1:200
Immunofluorescence staining of PC-3 cells with CSB-PA009143LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009143LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA009143LA01HU at dilution of 1:100
Western Blot
Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate
All lanes: GABRA4 antibody at 7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa