Immunofluorescence staining of PC-3 cells with CSB-PA009143LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009143LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA009143LA01HU at dilution of 1:100
Western Blot Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate All lanes: GABRA4 antibody at 7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
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