CDC45 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA005020ESR2HU
- Images (4)
| Article Name: | CDC45 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA005020ESR2HU |
| Supplier Catalog Number: | CSB-PA005020ESR2HU |
| Alternative Catalog Number: | CSB-PA005020ESR2HU-100UL, CSB-PA005020ESR2HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | CDC 45 antibody, CDC 45L antibody, CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody, CDC45 antibody, CDC45 cell division cycle 45 like antibody, CDC45 like antibody, CDC45 related protein antibody, CDC45_HUMAN antibody, CDC45L2 antibody, Cell division control protein 45 homolog antibody, Cell division cycle 45 like 2 antibody, Cell division cycle 45 like antibody, PORC PI 1 antibody, PORC-PI-1 antibody |
| Clonality: | Polyclonal |
| UniProt: | O75419 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | CDC45 |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000, IF:1:50-1:200 |
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005020ESR2HU at dilution of 1:100 |
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Western blot All lanes: CDC45 antibody at 0.3ug/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 61, 69 kDa Observed band size: 66, 69 kDa |
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Immunofluorescent analysis of Hela cells using CSB-PA005020ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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Immunoprecipitating CDC45 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA005020ESR2HU in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: CSB-PA005020ESR2HU (4ug) + K562 whole cell lysate (500ug) Lane 3: K562 whole cell lysate (20ug) |
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