CDC45 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA005020ESR2HU
Artikelname: CDC45 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA005020ESR2HU
Hersteller Artikelnummer: CSB-PA005020ESR2HU
Alternativnummer: CSB-PA005020ESR2HU-100UL, CSB-PA005020ESR2HU-50UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, IP, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: CDC 45 antibody, CDC 45L antibody, CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody, CDC45 antibody, CDC45 cell division cycle 45 like antibody, CDC45 like antibody, CDC45 related protein antibody, CDC45_HUMAN antibody, CDC45L2 antibody, Cell division control protein 45 homolog antibody, Cell division cycle 45 like 2 antibody, Cell division cycle 45 like antibody, PORC PI 1 antibody, PORC-PI-1 antibody
Klonalität: Polyclonal
UniProt: O75419
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: CDC45
Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005020ESR2HU at dilution of 1:100
Western blot
All lanes: CDC45 antibody at 0.3ug/ml
Lane 1: K562 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 66, 61, 69 kDa
Observed band size: 66, 69 kDa
Immunofluorescent analysis of Hela cells using CSB-PA005020ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating CDC45 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA005020ESR2HU in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA005020ESR2HU (4ug) + K562 whole cell lysate (500ug)
Lane 3: K562 whole cell lysate (20ug)