Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005020ESR2HU at dilution of 1:100
Western blot All lanes: CDC45 antibody at 0.3ug/ml Lane 1: K562 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 61, 69 kDa Observed band size: 66, 69 kDa
Immunofluorescent analysis of Hela cells using CSB-PA005020ESR2HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating CDC45 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA005020ESR2HU in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: CSB-PA005020ESR2HU (4ug) + K562 whole cell lysate (500ug) Lane 3: K562 whole cell lysate (20ug)
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