IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa
Western Blot Positive WB detected in: A549 whole cell lysate All lanes: AMOT antibody at 1.21ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 119 kDa Observed band size: 130 kDa
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