AMOT Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA001677ESR2HU
- Bilder (4)
| Artikelname: | AMOT Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA001677ESR2HU |
| Hersteller Artikelnummer: | CSB-PA001677ESR2HU |
| Alternativnummer: | CSB-PA001677ESR2HU-100UL, CSB-PA001677ESR2HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AMOT antibody, AMOT_HUMAN antibody, Angiomotin antibody, Angiomotin p130 isoform antibody, KIAA1071 antibody |
| Klonalität: | Polyclonal |
| UniProt: | Q4VCS5 |
| Puffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | AMOT |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
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IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa |
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Western Blot Positive WB detected in: A549 whole cell lysate All lanes: AMOT antibody at 1.21ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 119 kDa Observed band size: 130 kDa |
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