CD9 Recombinant Rabbit Monoclonal Antibody, Clone: [4C2], Unconjugated
Catalog Number:
BYT-ORB704168
- Images (7)
| Article Name: | CD9 Recombinant Rabbit Monoclonal Antibody, Clone: [4C2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704168 |
| Supplier Catalog Number: | orb704168 |
| Alternative Catalog Number: | BYT-ORB704168-25,BYT-ORB704168-50,BYT-ORB704168-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human CD9 (200-228aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29, CD9_HUMAN, CD9, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein (MRP-1), Tetraspanin-29 (Tspan-29), p24, CD9_MOUSE, CD9_RAT, |
| CD9 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [4C2] |
| Molecular Weight: | 25 kDa |
| UniProt: | P21926 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CD9 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:20-100 |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: Huvec (Human) Cell Lysate at 30 ug, Lane 6: A431 (Human) Cell Lysate at 30 ug, Lane 7: SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-CD9 (orb704168) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD. |
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IHC-P analysis of human laryngeal carcinoma using CD9 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse kidney using CD9 Monoclonal Antibody at 1:200 dilution. |
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