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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD9) Monoclonal Antibody, Unconjugated (orb704168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Testis (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Mouse) Lysate at 40 ug, Lane 5: Huvec (Human) Cell Lysate at 30 ug, Lane 6: A431 (Human) Cell Lysate at 30 ug, Lane 7: SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-CD9 (orb704168) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD. |
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IHC-P analysis of human laryngeal carcinoma using CD9 Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse kidney using CD9 Monoclonal Antibody at 1:200 dilution. |