SOD2 Recombinant Rabbit Monoclonal Antibody, Clone: [3G5], Unconjugated
Catalog Number:
BYT-ORB612222
- Images (6)
| Article Name: | SOD2 Recombinant Rabbit Monoclonal Antibody, Clone: [3G5], Unconjugated |
| Biozol Catalog Number: | BYT-ORB612222 |
| Supplier Catalog Number: | orb612222 |
| Alternative Catalog Number: | BYT-ORB612222-50,BYT-ORB612222-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human SOD2 (1-46aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | MNSOD, SOD2X1, SOD2X2, GC1, GClnc1, IPO-B, IPOB, MVCD6, Mn-SOD, lncRNA-GC1, Sod-2, SODM_BOVIN, SOD2, 1.15.1.1, SODM_HUMAN, SODM_MOUSE, SODM_RABIT, SODM_RAT, |
| SOD2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [3G5] |
| Molecular Weight: | 22 kDa |
| UniProt: | P04179 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SOD2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
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Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: mouse brain tissue lysate, Lane 2: SH-SY5Y cell lysate, Lane 3: mouse heart tissue lysate, Primary: Anti-SOD2 (orb612222) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD. |
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