SOD2 Recombinant Rabbit Monoclonal Antibody, Clone: [3G5], Unconjugated
Artikelnummer:
BYT-ORB612222
- Bilder (6)
| Artikelname: | SOD2 Recombinant Rabbit Monoclonal Antibody, Clone: [3G5], Unconjugated |
| Artikelnummer: | BYT-ORB612222 |
| Hersteller Artikelnummer: | orb612222 |
| Alternativnummer: | BYT-ORB612222-50,BYT-ORB612222-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human SOD2 (1-46aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | MNSOD, SOD2X1, SOD2X2, GC1, GClnc1, IPO-B, IPOB, MVCD6, Mn-SOD, lncRNA-GC1, Sod-2, SODM_BOVIN, SOD2, 1.15.1.1, SODM_HUMAN, SODM_MOUSE, SODM_RABIT, SODM_RAT, |
| SOD2 Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [3G5] |
| Molekulargewicht: | 22 kDa |
| UniProt: | P04179 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SOD2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
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Paraformaldehyde-fixed, paraffin embedded (human kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb612222) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: mouse brain tissue lysate, Lane 2: SH-SY5Y cell lysate, Lane 3: mouse heart tissue lysate, Primary: Anti-SOD2 (orb612222) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD. |
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