DMT1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB5976
Article Name: DMT1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB5976
Supplier Catalog Number: orb5976
Alternative Catalog Number: BYT-ORB5976-50,BYT-ORB5976-100,BYT-ORB5976-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human DMT1 (251-350/568aa)
Conjugation: Unconjugated
Alternative Names: AHMIO1, DCT1, DMT1, NRAMP2, mk, van, NRAM2_HUMAN, SLC11A2, NRAMP 2, Divalent cation transporter 1, Divalent metal transporter 1 (DMT-1), Solute carrier family 11 member 2, NRAM2_MOUSE,
DMT1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 62 kDa
UniProt: P49281
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SLC11A2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Divalent metal transporter 1, DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Divalent metal transporter 1, DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Sample: Adrenal glands (Mouse) Lysate at 40 ug, Primary: Anti-DMT1 (orb5976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 64 kD.
Western blot analysis of mouse Adrenal glands tissue using DMT1 antibody
Immunohistochemical analysis of Rat heart tissue using DMT1 antibody
Immunohistochemical analysis of Rat heart tissue using DMT1 antibody



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