Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Divalent metal transporter 1, DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Divalent metal transporter 1, DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DMT1) Polyclonal Antibody, Unconjugated (orb5976) at 1:400 overnight at 4C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Sample: Adrenal glands (Mouse) Lysate at 40 ug, Primary: Anti-DMT1 (orb5976) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 64 kD.
Western blot analysis of mouse Adrenal glands tissue using DMT1 antibody
Immunohistochemical analysis of Rat heart tissue using DMT1 antibody
Immunohistochemical analysis of Rat heart tissue using DMT1 antibody
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