Dnmt3a Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB500726
Article Name: Dnmt3a Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB500726
Supplier Catalog Number: orb500726
Alternative Catalog Number: BYT-ORB500726-50,BYT-ORB500726-100,BYT-ORB500726-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3a (51-150/912aa)
Conjugation: Unconjugated
Alternative Names: DNM3A_HUMAN, DNMT3A, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA (DNA MTase HsaIIIA | M.HsaIIIA), 2.1.1.37,
Dnmt3a Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 100 kDa
UniProt: Q9Y6K1
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: DNMT3A
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNMT-3 alpha) Polyclonal Antibody, Unconjugated (orb500726) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNMT-3 alpha) Polyclonal Antibody, Unconjugated (orb500726) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (orb500726) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Ovary (Mouse) Lysate at 40 ug, Lane 2: Ovary (Rat) Lysate at 40 ug, Primary: Anti-Dnmt3a (orb500726) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.
IHC-P analysis of rat lung using Dnmt3a Polyclonal Antibody at 1:200 dilution.
Western blot:Lane 1:Ovary (Mouse),Lane 2:Ovary (Rat),Primary:Anti-Dnmt3a at 1:1000 dilution.



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