Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNMT-3 alpha) Polyclonal Antibody, Unconjugated (orb500726) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DNMT-3 alpha) Polyclonal Antibody, Unconjugated (orb500726) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (orb500726) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Ovary (Mouse) Lysate at 40 ug, Lane 2: Ovary (Rat) Lysate at 40 ug, Primary: Anti-Dnmt3a (orb500726) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.
IHC-P analysis of rat lung using Dnmt3a Polyclonal Antibody at 1:200 dilution.
Western blot:Lane 1:Ovary (Mouse),Lane 2:Ovary (Rat),Primary:Anti-Dnmt3a at 1:1000 dilution.
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